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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene continues to be noticed in several tumors, which results in chromosome instability, and so may contribute to tumorigenesis. This protein was identified to associate with, and become linked to, the phosphorylation of NPAT protein (nuclear protein mapped on the ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a essential purpose in selling mobile-cycle progression from the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, are explained. Two more splice variants have been documented but specific nucleotide sequence data isn't still obtainable.
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Blocking peptides are peptides that bind precisely towards the focus on antibody and block antibody binding. These peptide usually is made up of the epitope regarded from the antibody.
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This guideline outlines further more feasible optimization of mobile and immunoassay parameters to guarantee the absolute best final results are...
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This guidebook outlines even further probable optimization of mobile and immunoassay parameters to ensure the best possible outcomes are...
Antibodies certain to the blocking peptide no more bind towards the epitope about the target protein. By evaluating the staining with the blocked antibody vs . the antibody alone, you can see which staining is precise.
Target Information Cyclin E belongs to your remarkably conserved cyclin relatives, whose customers are characterised by a remarkable periodicity in protein abundance with the mobile cycle. Cyclins operate as regulators of CDK kinases. Unique cyclins show unique expression and degradation styles which lead towards the temporal coordination of each and every mitotic occasion. Cyclin E sorts a fancy with and features to be a regulatory subunit of CDK2, whose exercise is required for mobile cycle G1/S changeover. This protein accumulates at the G1-S phase boundary which is degraded as cells progress via S section.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by equally antibodies...
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